Lindawati
University of Brawijaya, Indonesia
Title: Effect of physical exercise to FFA, pancreatic beta cell function and expression of GLUT4 in male Wistar mice type-2 with diabetes mellitus
Biography
Biography: Lindawati
Abstract
The purpose of this study is to see the effect of chronic moderate intensity physical exercise on Free Fatty Acids (FFA), function of pancreatic beta cells and expression of GLUT4 in male Wistar mice type-2 diabetes mellitus. This study uses 10 male Wistar rats of type-2 diabetes mellitus divided into two groups, group-1 was treated sedentary, group-2 was given physical exercise treatment using a treadmill rat (rodent-treadmill). Physical exercise was carried out six days a week for 10 weeks, beginning with light intensity and it was increased gradually every two weeks until it reaches moderate intensity. Then fasting blood glucose samples, fasting insulin, FFA were taken for the first two times in the two groups to see the presence of dysfunction and damage to pancreatic beta cells measured using Homeostatic Model Assessment (HOMA) B cells. Calculation of GLUT4 expression was done using an immunohistochemical method and it was observed using confocal laser scanning microscope. The results of this study indicate that physical exercise is not significant to reduce blood glucose levels, reduce blood insulin levels and increase levels of FFA, but it significantly increases the expression of GLUT4 on the surface of cell membranes (p=0.017) and improve pancreatic beta cell function by increasing the value of HOMA B (p=0.005).